Whole-body bioluminescence imaging of T-cell response in PDAC models

Introduction The location of T-cells during tumor progression and treatment provides crucial information in predicting the response vivo . Methods Here, we investigated, using our bioluminescent, dual color, T-cell reporter mouse, termed TbiLuc, function murine PDAC growth checkpoint blockade with anti-PD-1 anti-CTLA-4. Using this model, could visualize surrounding microenvironment longitudinally. We used clones that formed tumors either high infiltration (immunologically ‘hot’) or low ‘cold’). Results Differences total bioluminescence be seen between ‘hot’ ‘cold’ TbiLuc mice. During see tumor-draining lymph nodes an increase on day 7 after treatment. Conclusions In current work, showed mice can to monitor